A Review of the Science of Low Template DNA Analysis
نویسنده
چکیده
From our detailed review we find that the science supporting the delivery of Low Template DNA (LTDNA) analysis is sound and that the three companies (the Forensic Science Service Ltd, LGC Forensics and Orchid Cellmark Ltd) providing this service to the Criminal Justice System have validated their processes in accord with accepted scientific principles using both 28 and 34 PCR cycles for extracts containing less than 200 picograms (pg) of DNA. At these levels, stochastic and inhibition effects have an impact upon the DNA profiles produced and all those involved in this process have established guidelines for profile interpretation. Work on interpretation is continuing and it is for the Forensic Science Regulator to monitor this and to bring about some standardisation in interpretation amongst all providers.
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